High-resolution Mass Spectrometry and Accurate Mass Measurements with Emphasis on the Characterization of Peptides and Proteins by Matrix-assisted Laser Desorption/Ionization Time-of-Ñight Mass Spectrometry
نویسندگان
چکیده
Fundamental concepts of high-resolution mass spectrometry as it relates to the issue of accurate mass measurements are presented. Important issues speciÐc to magnetic sector instruments, Fourier transform ion cyclotron resonance and time-of-Ñight (TOF) methods are discussed. Recent high-resolution TOF measurements on peptides and proteins, ionized by matrix-assisted laser desorption/ionization (MALDI), are presented and discussed in terms of basic concepts important to accurate mass measurements. 1997 by John Wiley & Sons, Ltd. (
منابع مشابه
Accurate mass measurement of DNA oligonucleotide ions using high-resolution time-of-flight mass spectrometry.
Matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI) time-of-flight mass spectrometry (TOFMS) play an essential role in the analysis of biological molecules, not only peptides and proteins, but also DNA and RNA. Tandem mass spectrometry used for sequence analysis has been a major focus of technological developments in mass spectrometry, but accurate mass measure...
متن کاملMALDI-TOF MS Coupled with Collision-Induced Dissociation (CID) Measurements of Poly(methyl methacrylate)
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) is one of themost fundamental analysis techniques for proteins, peptides, and synthetic polymers to date. Along with field desorption (FD), fast atom bombardment (FAB), laser desorption coupled with time-of-flight (LD-TOF), secondary ion mass spectrometry coupled with time-of-flight (SIMS-TOF), and electr...
متن کاملMatrix-assisted laser desorption ionization--time of flight mass spectrometry of lipopeptide biosurfactants in whole cells and culture filtrates of Bacillus subtilis C-1 isolated from petroleum sludge.
An innovative method was developed for rapid sensitive detection and efficient structural characterization of lipopeptide biosurfactants by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry by using whole microbial cells and crude culture filtrates as targets in combination with surface tension measurements. This was done for a bacterial strain that was is...
متن کاملTissue Profiling by Mass Spectrometry
Matrix-assisted laser desorption ionization mass spectrometry (MALDI MS) has become a valuable tool to address a broad range of questions in many areas of biomedical research. One such application allows spectra to be obtained directly from intact tissues, termed “profiling” (low resolution) and “imaging” (high resolution). In light of the fact that MALDI tissue profiling allows over a thousand...
متن کاملSolvent-free mass spectrometry for hydrophobic peptide sequence analysis and protein conformation studies.
The breakthrough in soft ionization methods for biopolymers, electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI) (1,2), revolutionized mass spectrometry (MS). As a result of these effective bioanalytical technologies, the entire field of proteomics changed, and the use of mass spectrometers for protein identification by accurate mass measurements or de novo seq...
متن کامل